DEL screening with Salipro®
Salipro®-drug targets for discovery of small molecules by DNA-encoded library
DEL screening with
Salipro®
DNA-encoded library (DEL) screening is a cutting-edge method for drug discovery. It involves creating libraries of small molecules tagged with DNA barcodes. These libraries are screened against target proteins, allowing rapid identification of potential drug candidates.
Due to their complex nature, membrane proteins such as GPCRs, Ion Channels or SLC transporters can however be very challenging to use in DEL screenings. The Salipro® technology facilitates DEL screening of membrane proteins by maintaining their native structure and stability, thereby providing new possibilities for the development of novel drugs against challenging targets
DEL screening with Salipro®
Hit validation (e.g. SPR) with Salipro®
Hit optimization (e.g. cryoEM) with Salipro®
New pre-print combining DEL, SPR, CryoEM and functional data against a membrane protein target
In this study, Salipro collaborated with AstraZeneca, DynaBind and University of Copenhagen to demonstrate a complete workflow for discovering and characterizing novel modulators of the membrane protein PANX1, combining
Detergent-free DEL screening: Identification of PANX1 binders using DNA‑Encoded Library technology
SPR validation: Biophysical confirmation of binding on the same Salipro‑stabilized target
Cryo‑EM analysis: Structural insights into the Salipro–PANX1–compound complex
Functional assays: Electrophysiological validation showing functional inhibition of PANX1